ETF1 anticorps

N° du produit ABIN2720568

L’anticorps Souris Monoclonal anti-ETF1 a été validé pour WB et FACS. Il convient pour détecter ETF1 dans des échantillons de Humain.

Aperçu rapide pour ETF1 anticorps (ABIN2720568)

Antigène: ETF1 (Eukaryotic Translation Termination Factor 1 (ETF1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ETF1 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS)

Clone: 2E3

Détail du produit anti-ETF1 anticorps

Attributs du produit: Homo sapiens eukaryotic translation termination factor 1 (ETF1)

Purification: Purified from mouse ascites fluids by affinity chromatography

Immunogène: Full length human recombinant protein of human ETF1 (NP_004721) produced in HEK293T cell.

Isotype: IgG2a

Information d'application

Indications d'application: WB 1:500, FLOW 1:100,

Commentaires:

The concentration of the product may vary between diferrent lots.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5-1.0 mg/mL

Buffer: PBS (PH 7.3) containing 1 % BSA, 50 % glycerol and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Détails sur ETF1

Antigène: ETF1 (Eukaryotic Translation Termination Factor 1 (ETF1))

Autre désignation: ETF1

Sujet: Termination of protein biosynthesis and release of the nascent polypeptide chain are signaled by the presence of an in-frame stop codon at the aminoacyl site of the ribosome. The process of translation termination is universal and is mediated by protein release factors (RFs) and GTP. A class 1 RF recognizes the stop codon and promotes the hydrolysis of the ester bond linking the polypeptide chain with the peptidyl site tRNA, a reaction catalyzed at the peptidyl transferase center of the ribosome. Class 2 RFs, which are not codon specific and do not recognize codons, stimulate class 1 RF activity and confer GTP dependency upon the process. In prokaryotes, both class 1 RFs, RF1 and RF2, recognize UAA, however, UAG and UGA are decoded specifically by RF1 and RF2, respectively. In eukaryotes, eRF1, or ETF1, the functional counterpart of RF1 and RF2, functions as an omnipotent RF, decoding all 3 stop codons (Frolova et al., 1994 [PubMed 7990965]).

Poids moléculaire: 48.9 kDa

ID gène: 2107

NCBI Accession: NM_004730

HGNC: 2107